The effect of temperature on respiration

Every living thing respires in some government agency. there are two forms of public discussion, aerobic and anaerobic. Areobic ventilation only when takes place in the presence of oxygen and is a far more efficient way for complexed organisms to convert carbohydrates into energy. There are four stages in aerobic respiration, Glycolysis, the middleman reaction, Krebs cycle and oxidative phosphorylation. Anaerobic respiration is far less efficient than aerobic, but can produce low yields of energy quickly. T6he over totally equivalence for respiration isC6H12O6 + 6O2 6CO2 + 6H2O + energyA respiratory quotient (RQ) is used to show what substrate is existence used in respiration, it can withal show if anaerobic respiration is occurring. The following equations can be used to calculate RQRQ = batch of carbon dioxide given out in unit of time book of account of oxygen taken in unit of timeOrRQ = moles or molecules of carbon dioxide given outMoles or molecules of oxygen taken inPredictionI predict that an amplification in temperature will increase the respiration rate. The increase will only take place until a certain temperature is r individuallyed, the respiration rate will then last to come after that temperature. I think that the respiration rate will start to decrease after 50 C. This is because as the temperature increases, the enzymes and substrate molecules involved in respiration will gain more activation energy. This means that the enzymes and substrate are more likely to collide, and a reaction to take place. I think that respiration will reach its optimum rate at about 40 C, this is because enzymes work best at about this temperature, that is only providing that another factor, such as oxygen available, does not become a limiting factor.Preliminary workTo canvas the effects of temperature on respiration a respirometer wil be used. A respirometer consists of two essay thermionic vacuum tubes, an tryal test tube and a control tube, and a capillary U-tube. The experimental tube contains soda-lime solvent with a wire gauze platform over it, with non-vertebrates in it. The control tube also contains soda-lime, with a wire gauze preceding(prenominal) it, with glass beads in it. Both tubes are sealed and tie to the u-tubing containing manometer fluid.A respirometer is suitable for measuring the rate of oxygen consumption of subatomic terrestrial invertebrates. The Carbon dioxide produced in respiration is absorbed by a suitable chemical such as soda-lime or Potassium hydrated oxide solution. The amount of oxygen used can be measured by reading the direct of manometer fluid against the scale. using a piddle vat alters the temperature.We did a test run using this equipment germinating peas were used instead of olive-sized invertebrates. Only one test was completed, this was at room temperature no repeats were made. This was repayable to time restrictions. This test run taught us how to set up the equipment. We compared our results gained to extension phone book values and decided that they were reasonably reliable.Variables* The surrounding atmospheric temperature of the water bath must perch constant to prevent anomalous results being obtained.* The atmospheric pressure must remain constant this is ok because the experiment will take place in the same room all of the time.* A control tube is used to compensate in small changes in atmospheric pressure.* The same volume and concentration of NaOH must be used in every experiment this is to prevent more or less CO2 being absorbed.* The mass of the invertebrates and the number of invertebrates must remain constant to prevent differences in respiration rates occurring.Five incompatible temperature inputs will be used, these temperatures will be 10 C, 20 C,30 C, 40 C and 50 C. These temperatures provide a good pad for which varying respiration rates can occur. The enzymes involved in respiration will be working very slowly at both 10 C and 50 C. The optimal temperature for respiration can also be found using these temperatures. These changes in temperature will not do any harm to the invertebrates, by over heating them. each(prenominal) test will be repeated three times to gain an medium result and to remove any anomalous results as far as possible.Apparatus* Two large test tubes* Sodium Hydroxide solution* Two test tube bungs, with two holes in each* 4 capillary tubes* 1 capillary u-tube containing manometer fluid* 1Cm syringe* 8 maggots* field glass beads* 2 small pieces of wire gauze* 1 kip down clip* 1 three way tap* Stopwatchmethod acting1. Set up the equipment as shown in the diagram above2. Using the syringe, adjust the direct of manometer fluid in the u-tubing to ensure that it is the same level at both sides3. Seal the screw clip and record the level of manometer fluid every two minutes, over a period of 10 minutes.4. repeat step 3 for the temperatures of 10 C, 20 C(room temperature), 30 C, 40 C and 50 C. These temperatures can be reached by using any a water bath or crushed ice5. Each test must be repeated 3 times.6. Record each result in a table and work out the average. physical exertion the results to calculate the RQ values.Risk Assessment* The NaOH is corrosive, so it must be kept away from the invertebrates. Saftey goggles and a lab coat must be worn to protect eyes and clothes from NaOH.* Living creatures must be treated with respect* Any spillages must be mopped up right off to prevent accidents occurring.